Development and Clinical Application of Liquidchip Luminex Assay in the Detection of Epidermal Growth Factor Receptor and K-Ras Mutation
Development and Clinical Application of Liquidchip Luminex Assay in the Detection of Epidermal Growth Factor Receptor and K-Ras Mutation
Journal of Immunobiology (Hilaris Publishing S.L) has announced almost 50% discount on article processing charge to commemorate its 12th Anniversary.
Journal of Immunobiology is an open access, peer-reviewed journal that focuses and welcomes submissions on all aspects of Immunobiology, Immunology, Autoimmunity, Innate Immunity, Adaptive Immunity, immunization, Immunogenetics, Immune Disorders, Immunodeficiency, Immune inflammation, Immunoinformatics, Clinical Immunology, Allergy & Immunology, Clinical & Vaccine Immunology, Inflammatory Disorders, Autoimmune Disorders, Immunotherapy and Immunopathology of infectious, allergic and autoimmune disease etc.
It gives us great pleasure to announce the call for paper on the occasion of 10th Anniversary of the Journal at special and hefty discount of up to 50 % on one-time article processing charge. Prospective academicians and scientists are encouraged to utilize this opportunity to get their articles reviewed, processed and published at relatively faster pace and at lower charges. In addition to this, the authors who publish with us during the year-long celebrations will also be eligible for academic awards recommended by the editorial panel.
The Archive page contains wide variety of articles such as Research / Review / Case reports / short communication / Mini review / Prospective / Letter to Editors Etc. We would like introduce a Review article which has been spread to the widest audience of experts; and thus increased in readership, citations and altimetry score.
Abstract: The success of molecular targeted cancer therapy relies on the accurate detection of the mutated gene. We attempted to develop a rapid, accurate, high sensitive and specific liquid chip luminex method for the detection of EGFR and K-ras mutation, both of which are important biomarkers for the personalized treatment of advanced lung cancer patients. Using the liquid chip technology, we developed a luminex system by combining PCRLDR (Polymerase Chain Reaction- Ligase Detection Reaction) with luminex platform for the detection of EGFR and K-ras mutation. To verify the clinical application of this liquid chip luminex system, we compared its detection results with those from the gold standard sequencing method through analysis of 100 patients. The developed luminex system showed high flux, sensitivity and specificity for EGFR and K-ras gene mutation detection. Compared with sequencing for the EGFR and K-ras gene mutation detection, this luminex system showed no obvious difference in the mutation rates among different ages, histological classification and TNM stages. However, for the exon 21 L585R and exon 19 (including the E746-A750 deletion mutant), the luminex method showed even more effective and specificity and demonstrated obvious difference to sequencing (p<0.05). Our liquid chip luminex system has a wide prospect of clinical application, especially for the detection of EGFR exon 21 L585R and 19 and can be used for early screening and individual therapy of patients with lung cancer.
To have a glance at the full length manuscript, you can visit us at our archive page and Currently, Journal’s Archive is holding not only normal issues but also focusing on special issues. The purpose of special issues is to publish the most exciting research with respect to the subjects of Immunology / Immunobiology and to provide a rapid turn-around time regarding reviewing and publishing, and to publicize the articles freely for research, teaching and reference purposes. Submit manuscript of your research articles or special issue articles online through manuscript submission or forwarded to the Editorial Office at immunobio@immunologyjournals.org
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Journal of Immunobiology